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Reconstitution calculator
Reference

Supplies

What you need on the bench to prepare and store a research peptide, and why each item is on the list. Every reason cites a published source.

Supplies list

The items a reconstitution and storage routine uses, and why each one is on the list. Every reason cites a source. A supplier link appears only where an item is sold by a partner or a retailer listing is live.

Diluents and solvents

  • Bacteriostatic water (BAC water), USP

    Why it is needed: USP bacteriostatic water contains benzyl alcohol and is labelled only as a diluent; CDC: date a multiple-use vial and discard it within 28 days. Source: labeling.pfizer.com

  • Sterile water, USP (preservative-free)

    Why it is needed: Preservative-free diluent; EGRIFTA SV's label names sterile water with immediate use, EGRIFTA WR's names bacteriostatic water. Follow the product label. Source: dailymed.nlm.nih.gov

  • Acetic acid 0.6% research solvent, 10 mL

    Why it is needed: Dilute acetic acid is a documented laboratory solvent for dissolving basic peptides; the solvent named in the product documentation takes precedence. Source: nibsc.org

Syringes

  • U-100 insulin syringe, 0.3 mL (30 units)

    Why it is needed: U-100 syringes are marked in units, 100 units per mL; a 0.3 mL barrel has 0.01 mL graduations (spec: BD 0.3 mL, 31 G x 5/16 in). Source: delasco.com

  • U-100 insulin syringe, 0.5 mL (50 units)

    Why it is needed: U-100 means 100 units per mL, so a 0.5 mL barrel spans 50 units; check the unit markings before reading a draw volume. Source: medlineplus.gov

  • U-100 insulin syringe, 1 mL (100 units)

    Why it is needed: Standard U-100 insulin syringes hold 1 mL (100 units); each small notch on a 1 mL syringe is 1 unit. Source: medlineplus.gov

Needles

  • Needle gauge and length (e.g. 31 G, 5/16 in or 6 mm)

    Why it is needed: Manufacturer spec for a U-100 insulin syringe: 31 G needle, 5/16 in (8 mm) length, with a 6 mm version also listed. Gauge number rises as diameter falls. Source: delasco.com

Swabs and wipes

  • Alcohol prep pads (isopropyl)

    Why it is needed: CDC lists alcohol wipes among supplies for the medication preparation area; MedlinePlus: wipe the vial top with an alcohol wipe. Source: cdc.gov

Sharps disposal

  • FDA-cleared sharps disposal container

    Why it is needed: FDA recommends used needles and sharps go immediately into an FDA-cleared sharps disposal container, made of rigid plastic with a fill line. Source: fda.gov

Storage

  • Digital refrigerator thermometer / data logger

    Why it is needed: CDC's vaccine storage toolkit says to use digital data loggers for routine storage temperature monitoring and to keep a backup. Source: cdc.gov

  • Small refrigerator (dedicated)

    Why it is needed: Bachem: lyophilised peptide stored tightly closed below -15 C for long term; a 4 C refrigerator is acceptable for short-term use. Source: bachem.com

  • Vial storage case or box with desiccant

    Why it is needed: Bachem: peptides are hygroscopic; keep the lyophilisate in a tightly closed container and reseal tightly after use. Source: bachem.com

Labelling

  • Vial labels and label printer

    Why it is needed: CDC: a multiple-use vial should be dated when opened and discarded within 28 days, so a dated label on each vial and diluent bottle records the date. Source: cdc.gov

Other

  • Nitrile examination gloves (non-powdered)

    Why it is needed: Examination gloves are disposable Class I devices worn on the hand as a barrier against cross-contamination (21 CFR 880.6250). Source: law.cornell.edu

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