Laboratory calculator

AOD-9604 Reconstitution Calculator (5 mg vial)

Worked example

Worked example: a 5 mg vial of AOD-9604 reconstituted with 2 mL of bacteriostatic water has a concentration of 2.5 mg/mL. A 250 mcg example amount is a 0.1 mL aliquot, which reads 10 units on a U-100 syringe, and the vial holds 20 aliquots of that size.

Reconstitution calculator

Vial size (mg)

Bacteriostatic water added (mL)

Target amount per draw (mcg)

Syringe (U-100)

Sizes in the Pepcore catalogue10 mg

Concentration: 2.5 mg/mL
Volume to draw: 0.1 mL
Units on a U-100 syringe: 10 units
Draws per vial: 20

How it works

Concentration = vial mass / water volume: 5 mg / 2 mL = 2.5 mg/mL.
Aliquot volume = target amount / concentration: 0.25 mg / 2.5 mg/mL = 0.1 mL.
Units on a U-100 syringe = mL x 100 = 10.
Aliquots per vial = vial mass in mcg / target amount = 5,000 / 250.

Frequently asked questions

How much water does a 5 mg vial of AOD-9604 need for 2.5 mg/mL?

Concentration is vial mass divided by water volume: 5 mg / 2 mL = 2.5 mg/mL. Change the water volume in the calculator to see how the concentration and the syringe reading move together.

How many units on a U-100 syringe is a 250 mcg example amount of AOD-9604?

At 2.5 mg/mL a 250 mcg example amount is 0.1 mL, which is 10 units because 1 unit on a U-100 syringe equals 0.01 mL.

How many 250 mcg aliquots are in a 5 mg vial of AOD-9604?

5 mg is 5,000 mcg, and 5,000 mcg divided by 250 mcg gives 20 aliquots, before allowing for volume lost to priming and syringe dead space.