Laboratory calculator
MOTS-c Reconstitution Calculator (5 mg vial)
Worked example
With 5 mg of MOTS-c in 2 mL, the concentration is 5 / 2 = 2.5 mg/mL. A 250 mcg example amount (0.25 mg) needs 0.25 / 2.5 = 0.1 mL, or 10 units on a U-100 syringe, and 5,000 mcg divided by 250 mcg gives 20 aliquots per vial.
Reconstitution calculator
Sizes in the Pepcore catalogue10 mg
- Concentration
- 2.5 mg/mL
- Volume to draw
- 0.1 mL
- Units on a U-100 syringe
- 10 units
- Draws per vial
- 20
How it works
- Concentration = vial mass / water volume: 5 mg / 2 mL = 2.5 mg/mL.
- Aliquot volume = target amount / concentration: 0.25 mg / 2.5 mg/mL = 0.1 mL.
- Units on a U-100 syringe = mL x 100 = 10.
- Aliquots per vial = vial mass in mcg / target amount = 5,000 / 250.
Frequently asked questions
What concentration does 2 mL of water give in a 5 mg vial of MOTS-c?
Concentration is vial mass divided by water volume: 5 mg / 2 mL = 2.5 mg/mL. Change the water volume in the calculator to see how the concentration and the syringe reading move together.
What does a 250 mcg example amount of MOTS-c read on a U-100 syringe?
At 2.5 mg/mL a 250 mcg example amount is 0.1 mL, which is 10 units because 1 unit on a U-100 syringe equals 0.01 mL.
How many aliquots of 250 mcg does a 5 mg MOTS-c vial yield?
5 mg is 5,000 mcg, and 5,000 mcg divided by 250 mcg gives 20 aliquots, before allowing for volume lost to priming and syringe dead space.