Laboratory calculator
NAD+ Reconstitution Calculator (100 mg vial)
Worked example
Worked example: a 100 mg vial of NAD+ reconstituted with 5 mL of bacteriostatic water has a concentration of 20 mg/mL. A 250 mcg example amount is a 0.0125 mL aliquot, which reads 1.25 units on a U-100 syringe, and the vial holds 400 aliquots of that size.
Reconstitution calculator
Sizes in the Pepcore catalogue500 mg
- Concentration
- 20 mg/mL
- Volume to draw
- 0.0125 mL
- Units on a U-100 syringe
- 1.3 units
- Draws per vial
- 400
How it works
- Concentration = vial mass / water volume: 100 mg / 5 mL = 20 mg/mL.
- Aliquot volume = target amount / concentration: 0.25 mg / 20 mg/mL = 0.0125 mL.
- Units on a U-100 syringe = mL x 100 = 1.25.
- Aliquots per vial = vial mass in mcg / target amount = 100,000 / 250.
Frequently asked questions
What concentration does 5 mL of water give in a 100 mg vial of NAD+?
Concentration is vial mass divided by water volume: 100 mg / 5 mL = 20 mg/mL. Change the water volume in the calculator to see how the concentration and the syringe reading move together.
What does a 250 mcg example amount of NAD+ read on a U-100 syringe?
At 20 mg/mL a 250 mcg example amount is 0.0125 mL, which is 1.25 units because 1 unit on a U-100 syringe equals 0.01 mL.
How many aliquots of 250 mcg does a 100 mg NAD+ vial yield?
100 mg is 100,000 mcg, and 100,000 mcg divided by 250 mcg gives 400 aliquots, before allowing for volume lost to priming and syringe dead space.