Laboratory calculator
NAD+ Reconstitution Calculator (250 mg vial)
Worked example
Worked example: a 250 mg vial of NAD+ reconstituted with 10 mL of bacteriostatic water has a concentration of 25 mg/mL. A 250 mcg example amount is a 0.01 mL aliquot, which reads 1 unit on a U-100 syringe, and the vial holds 1,000 aliquots of that size.
Reconstitution calculator
Sizes in the Pepcore catalogue500 mg
- Concentration
- 25 mg/mL
- Volume to draw
- 0.01 mL
- Units on a U-100 syringe
- 1 unit
- Draws per vial
- 1000
How it works
- Concentration = vial mass / water volume: 250 mg / 10 mL = 25 mg/mL.
- Aliquot volume = target amount / concentration: 0.25 mg / 25 mg/mL = 0.01 mL.
- Units on a U-100 syringe = mL x 100 = 1.
- Aliquots per vial = vial mass in mcg / target amount = 250,000 / 250.
Frequently asked questions
What concentration does 10 mL of water give in a 250 mg vial of NAD+?
Concentration is vial mass divided by water volume: 250 mg / 10 mL = 25 mg/mL. Change the water volume in the calculator to see how the concentration and the syringe reading move together.
What does a 250 mcg example amount of NAD+ read on a U-100 syringe?
At 25 mg/mL a 250 mcg example amount is 0.01 mL, which is 1 unit because 1 unit on a U-100 syringe equals 0.01 mL.
How many aliquots of 250 mcg does a 250 mg NAD+ vial yield?
250 mg is 250,000 mcg, and 250,000 mcg divided by 250 mcg gives 1,000 aliquots, before allowing for volume lost to priming and syringe dead space.